Troubles with leukocyte adhesion - what to do? - (Oct/04/2013 )
I am working with living leukocytes, which I use for surface-enhanced Raman spectroscopy (SERS) and it is crucial that the cells are as still as possible in order for me to create a spectral image. The leukocytes are directly extracted from human donor blood and then fronzen in liquid nitrogen (I am not doing this procedure myself).I have been trying to attach them to the well bottom (glass) by using poly-D-lysine >300.000 kDa MW, but without success.
The protocol I use is the one provided by Sigma Aldrich (general, not cell type specific):
Are you sure to complete lysis of RBCs? sometimes remainig RBCs in media cause troubles in cell adherence.
Hi, thank you for you answer!
If you mean red blood cells, I cannot tell as I receive those leukocytes from another laboratory. I will ask the person preparing them however. How are leukocytes different in terms of adherence from any other common cell lines for instance? What properties should one take into consideration in general when dealing with leukocytes?