Contamination.... - (Oct/02/2013 )
Greetings everyone. So my co worker and I ordered some bacteria from Carolina, and the lately our Staphylococcus epidermidis has bacillus subtilis contamination... We are rehydrating our bacteria into steril nutrient agar and using only sterile syringes. I havent been working at this college laboratory very long but I wanted to take the initiative and look this up myself maybe there is something I am not doing right. Thank you.
What protocol demands the use of syringes?
Is your isolate in a sterium bottle? That would be a reason to use a syringe...are you having to work anaerobically?
Sorry - of course you meant from the original vial rehydrated, not subsequent subculture. .
I'd contact the supplier.