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How to get rid of bands in PCR negative control - (Sep/12/2013 )

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As phage434 mentioned, use something other than 70% ethanol - perhaps DNA away or 10 % bleach.

 

What is your downstream application with the amplicon? Do you run gels/visualize the PCR products in the same lab you set up the PCR? Are there others working with the same target?

 

There could be an issue with your work flow causing contamination of your wells with amplicon. This could also explain why you don't see contamination in every no template control (which should occur if it is a reagent issue).

 

As a simple test, perform a PCR reaction targeting a different gene in the same organism (preferably a target which is not regularly amplified!). If your negatives are clean, the contamination issue is likely due to amplicon resulting from your work flow.

-biothreat-
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