Glucose levels from plasma in mice - (Sep/04/2013 )
We are looking to examine blood glucose levels in mice plasma. I know that ideally this would be done by serial blood draws, but if blood glucose was sky high we should see something at a single time point, so thats what we are planning to do; take blood from the mice, euthanise and then measure the glucose? Thats possible right?
Also is there any particular recommended glucose assay kit?
Glucose assay in microtiter plate (by Zagami)
Buffer enzyme reagent: dissolve 15 U/ml of GOD from Aspergillus niger in 1 liter potassium phosphate buffer 0.5 M at pH 7.2 (mix 800 ml of 0.5 M KH2PO4 with 200 ml 0.5 M KH2PO4)
Trinder reagent: dissolve 45 mM phenol, 7.5 mM 4-amino antipyrine, 42 U/ml peroxidase and 92 mM NaN3
Working solution: mix 1 volume Trinder reagent with 5 volume Buffer enzyme reagent.
Dispense in MTP well 100 µl of working reagent and 3 µl sample (serum/plasma, urine or other biological fluids) and add 140 µl of dH2O. Read Abs after 8 min. between 500 to 520 mm and compared it with calibrators having known glucose concentrations.