Protocol Online logo
Top : New Forum Archives (2009-): : -Botany and Ecology-

Orchid Chromosomes - (Jul/23/2013 )

Hi All,

Has anyone dealt with Orchid chromosomes? Which pre treatment is effective for root tips 0.002M 8 Hydroxy quinoline or PDB.

I have to count chromosomes from orchid plants. Please share your experience dealing with the roots and chromosome preparation etc.

Thanks

N

-dr_kaur66-

I asume that You mine internet for this solution and You havent found one in any paper.

 

In animals it is easy to count chromosomes during the cell division. They gathering together and they are easy to distinguish. Maybe the same method will work in plant division. 

 

I would write to people who work on plant genetics especially those who work on orchids. I know one person who work on that but I don't know if he is still in this topic. His name is Grzegorz Wojtczak. He is working on plant-microbial interactions.

 

I thik that counting chromosomes in plants is easy because of great impact of number of chromosomes sets on species differentiation, species emerging and potentially usage in agroengineering.

 

Good luck in Your research,

Allies

-Allies2000-

Many Thanks for your suggestions Allies. And would appreciate if you can contact the person who works on Orchids.

Actually I did my PhD on plant chromosomes and have quite a bit of ideas how to tackle these but haven't particularly worked with Orchids and I guess it'll be hard as they have very small sized chromosomes and usually above 40. so any suggestions related to handling of Orchids would be a great help.

 

Thanks again

 

N

-dr_kaur66-

Many Thanks for your suggestions Allies. And would appreciate if you can contact the person who works on Orchids.

Actually I did my PhD on plant chromosomes and have quite a bit of ideas how to tackle these but haven't particularly worked with Orchids and I guess it'll be hard as they have very small sized chromosomes and usually above 40. so any suggestions related to handling of Orchids would be a great help.

 

Thanks again

 

N

You did your work on plant chromosomes ? uh?

Didnt you go to some international confrences where you could meet some people that work on plants/orchids? Cant you contact them?

 

You have to be very Lucky to find someone here that worked on them... so I think it might be a better idea to look around for people that work(ed) on them... you have no contacts?

-pito-