Protocol Online logo
Top : New Forum Archives (2009-): : Tissue and Cell Culture

Ca n Mg free PBS - (Jul/01/2013 )

Can any one tell me how to prepare Ca++ and Mg++ free PBS for cell isolation


Dulbecco’s phosphate-buffered saline (Dulbecco’s PBS)
2.7 mM KCl
1.5 mM KH2PO4
136.9 mM NaCl
8.9 mM Na2HPO4•7H2O


Thanks bob1