Protein purification from greenish supernatant of Pichia pastoris - (Jun/28/2013 )

You say you need to express in Pichia for the low cost...I'm not sure what the alternatives are, but it would seem to me your boss if paying you quite a bit money for the time it has taken you to trouble shoot this problem without much success and poor yield. Sometimes investing a little extra $$ upfront will save lots of time and $$'s down the road.

I had a boss in grad school that insisted I spend weeks developing an assay or protocol with home-made materials so that he wouldn't have to spend the $200 for the kit. So instead of paying $200 and having the results in 2 days, he paid me for four weeks of labor and time before getting any resuls. Basically, my boss ended up losing both time and money in the whole process, thinking he just saved a bundle by not buying the kit. This is what I would pitch to your boss if this were my project.