Pelleting cells by gentle centrifugation for addition to media - (Mar/19/2013 )

They water was originally taken as deep water. I have incubated it under methane for 3 months at various concentrations. I am now transfering cells to media to grow them in ASW under methane headspace. I'm not too concerned about losing too many cells just want to make sure that I get enough and if I should be able to see them when I pellet them. The previous comment seems as if it is going to take a really long time to pellet the cells.

Deep sea origin but cultivated at atmospheric pressure?

Yes. well they are from in the water column they are aerobic. If I try filtering also, we do vacuum filtration. Wont this be hard on the bugs?

Aerobic and deep sea origin? Are you saying they're facultative?