Qiagen column eluted DNA gives higher concetration than the original sample - (Feb/09/2013 )
Hi Carel, This is very nice of you to take the time to help. Great way to approcach the calculations. I think you are rihgt. I'm going to have to check a few things to make sure nothing is coming from contaminants low 260/280 ratio for example (However unlikely). I also need to run the gel, I am a lttle afraid of the conformational changes of larger DNA fragments (i.e gDNA samples). The fluorescent molecules access to DNA fragmetns (Specially the way PicoGreen binds to DNA) can also be a factor. I know two out of three samples I tested had issues with degradation. I still have to run the gel and check the bands.
My pipetted volumes were above 5 ul, it's unlileky that this is the issue. I agree on the elution volume collected from columns. It's hardly 100 ul, however it's usually around 90-95 ul. Thanks again for your help. I really appreciate it.