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Dam Dcm Negative Strain - (Jan/08/2013 )

I red that methylated DNA has less tranformation efficiency than unmethylated. How can i get my plamid unmethylated?


You can use strains of bugs without the methyl transferases. These strains will be Dam-/Dcm-. I don't know of any way to do this post extraction if that is what you are asking.


Can you give some example like JM110 or others? Which strain is most frequently used in the Labs?