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collagen coating of transwell inserts - (Jan/08/2013 )


I seeded the Caco2 cells in transwell inserts to do a permeability assay in 21 days. I just forgot to suck off the collagen solution that I had used for coating, before I seed the cells. Will it make a big problem for me or I can still count on the cells? I don't know how seriously it affects the forming of cell monolayer and the permeability results.
Thanks in advance!


I think just remove them, wash them and re-culture to get an even monolayer.


Thank you!