Cisplatin affect of HeLa - (Jan/06/2013 )
I have tested apoptotic activity for Cisplatin againts HeLa cells using RayBio® Human Apoptosis Antibody Array Kit.
The results is confusing where expression of p53 and caspase 3 decreases overtime but level of XiAP decreases dramatically.
So, does it shows apoptosis?
Cisplatin should increase p53 expression and decrease XIAP expression, while increasing the amount of cleaved caspase-3. Based on the Raybiotech website, it seems they're array is detecting the apoptosis-induced cleavage product of caspase-3: http://www.raybiotech.com/products/antibody-arrays/g-series-en/apoptosis-arrays/ although you should try to confirm that their antibody isn't detecting full-length and cleaved forms. I suspect you are studying "noise".