Avoiding PTM Actylation of rProtein - (Jan/03/2013 )
How can İ prevent acylation (Lys) of my recombinant protein in E. coli or Mammalian cells?
Could you treat the cells with an acyltransferase inhibitor? They are available commericially.
This option also came in my mind but to knockout the gene would be Lethal i guess. In mammalian maybe one could use stable cell lines transfected with shRNA, or?
Is there a way to screen a inhibitor collection?
Im not quite following. You fear that if you use an acyltransferase inhibitor you will "knock out" the gene/protein function? If you want to prevent acylation, these inhibitors seem like the logical choice. It will inhibit acylation of all proteins, but there will be a recommended dose to use that should maintain a majority of protein functions.
No of course inhibition will be happing on the protein level. I mean cutting out the gene will be lethal. But thanks for sharing your knowledge.