Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Biology

Avoid SDS in thermal lysis: whole blood? - (Jan/03/2013 )

Is there a way out to reduce frothing by avoiding SDS (or replacing with something that reduces frothing) in the lysis buffer for whole blood. I am trying to do lysis in a microfluidic channel with heating from the bottom and SDS (1%) causes a lot of frothing that becomes uncontrollable. If I remove SDS, it coagulates, Even 0.5% SDS lead to coagulation.


Pending on what you want to lysate, but for for PBMC separation I start with PBS (without Mg and Ca) with 2mM EDTA.
Hope you find it helpful!