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Barcoding PCR products - (Dec/11/2012 )

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"short DNA barcodes (4 - 6 nt)", what is that barcode type? I never saw it before. Does it similar to other linear and 2D link removed like QR Code or Code 128?

No spamming links - bob.

-harkstatemen-

harkstatemen on Fri Jan 18 03:58:55 2013 said:


"short DNA barcodes (4 - 6 nt)", what is that barcode type? I never saw it before. Does it similar to other linear and 2D link removed like QR Code or Code 128?

Nothing to do with conventional barcoding -this is a biology forum.

-bob1-

bob1 on Fri Jan 18 07:59:32 2013 said:


harkstatemen on Fri Jan 18 03:58:55 2013 said:


"short DNA barcodes (4 - 6 nt)", what is that barcode type? I never saw it before. Does it similar to other linear and 2D and here too like QR Code or Code 128?

Nothing to do with conventional barcoding -this is a biology forum.

at least the idea is the same...you have a product/article/item or or a species or a DNA sequence for what you want a unique identifier. this can be in the first case an unique set of parallel lines varying in width and spacing (classical barcode, text from wikipedia) or a unique set of nucleotides specific for your aim.

-hobglobin-

hobglobin on Fri Jan 18 16:31:29 2013 said:


bob1 on Fri Jan 18 07:59:32 2013 said:


harkstatemen on Fri Jan 18 03:58:55 2013 said:


"short DNA barcodes (4 - 6 nt)", what is that barcode type? I never saw it before. Does it similar to other linear and 2D and here like QR Code or Code 128?

Nothing to do with conventional barcoding -this is a biology forum.

at least the idea is the same...you have a product/article/item or or a species or a DNA sequence for what you want a unique identifier. this can be in the first case an unique set of parallel lines varying in width and spacing (classical barcode, text from wikipedia) or a unique set of nucleotides specific for your aim.

True, however I suspect that Harkstatemen is actually a spammer, but I was giving them the benefit of the doubt.

-bob1-

Just to update, I re ordered the primers, and it works fine. Possibly something wrong with the sequence of the earlier ones due to some error, somewhere. I didnt change any sequences coz I was sure about them, just re ordered from another company!

-GradMicro-

wow, so many good advice, I would rather take this Oh no - your link disappeared site.

No spamming links, thanks - bob.

-Tate-

Thanks again, but this is not the type of barcodes that are used in molecular biology...

-GradMicro-

Before amplifying the DNA of interest, we need to extract the DNA from the selective species first. This preliminary preperations are not so easy and is time consumable. But I want to inform that The FastPrep-24™ 5G Instrument is an efficiant one to extract DNA, RNA and proteins from different biological samples within 40 seconds.

 

Have anyone used it?

 

 

I am looking for a second opinion about this homogenizer.

you are from the company that sells it? 

You do not trust your own stuff than? A bit weird, no?

-pito-

 

 

Before amplifying the DNA of interest, we need to extract the DNA from the selective species first. This preliminary preperations are not so easy and is time consumable. But I want to inform that The FastPrep-24™ 5G Instrument is an efficiant one to extract DNA, RNA and proteins from different biological samples within 40 seconds.

 

Have anyone used it?

 

 

I am looking for a second opinion about this homogenizer.

you are from the company that sells it? 

You do not trust your own stuff than? A bit weird, no?

 

No. I am not from that company but I heared and was informed about it. I don't know what made you understand that i am from that company. I asked if anyone used it or not. I need a second opinion to install this instrument in my lab.

 

Surprisingly almost all your posts have a link to the MPbio company, which surely makes it a legitimate question if you are from this company.

And well here you write the MPbio instrument is an "efficient one", and it sounds like an advert, but then you tell you haven't used it and need more input to buy it?? Really a bit weird....

-hobglobin-
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