Protocol Online logo
Top : New Forum Archives (2009-): : Microbiology

Can medium containing starch be autoclaved? - (Nov/21/2012 )

Pages: Previous 1 2 3 4 

Ah! I see. Darn. 9g/100 mL puts my concentration at 9%. Per my calculations, I need to prepare 20%. So, the calculations that I did above need to be adjusted from 20% to 9% correct?

-SunsetSatine-

I went ahead and add 50 mL of DI water to the solution to dilute it down, and it all dissolved. Does this mean that my solution is still 10% concentration? Or given the solubility, is it actually at 9% concentration?

-SunsetSatine-

SunsetSatine on Wed Dec 12 22:00:22 2012 said:


I went ahead and add 50 mL of DI water to the solution to dilute it down, and it all dissolved. Does this mean that my solution is still 10% concentration? Or given the solubility, is it actually at 9% concentration?


You added 50ml to what?
WHat is your endvolume now?
10grams in 100ml or?

-pito-

Final volume was 100 mL

-SunsetSatine-

SunsetSatine on Thu Dec 13 17:15:05 2012 said:


Final volume was 100 mL


Wel then you have 10grams in 100Mml...

10% solution

-pito-

pito on Thu Dec 13 17:27:12 2012 said:


SunsetSatine on Thu Dec 13 17:15:05 2012 said:


Final volume was 100 mL


Wel then you have 10grams in 100Mml...

10% solution

But not if only 9g are soluble in 100 mL....at room temperature there should be a precipitate visible of the remaining gram.

-hobglobin-

hobglobin on Thu Dec 13 17:47:16 2012 said:


pito on Thu Dec 13 17:27:12 2012 said:


SunsetSatine on Thu Dec 13 17:15:05 2012 said:


Final volume was 100 mL


Wel then you have 10grams in 100Mml...

10% solution

But not if only 9g are soluble in 100 mL....at room temperature there should be a precipitate visible of the remaining gram.


I am assuming he was able to dissolve it completely.

But indeed, you are right, is there is precipitation then its not 10% rather 9%

-pito-
Pages: Previous 1 2 3 4