pre mRNA after total mRNA isolation? - (Nov/21/2012 )
I am studying different spliced isoforms from a particular gene by RT-qPCR. I get amplification from a particular intron. How can I know whether the amplification is coming from pre mRNA? Can one 'clean' somehow the mRNA from the pre mRNA?
Thank you.
-Niwrad-
pre-mrna is processed so quickly that it is highly unlikely to be present in sufficient quantities to interfere.
-mdfenko-
It can also come from the traces of DNA, that is far more likely. You can use DNAse to clen your sample.
-Trof-