Serum inactivation: - (Nov/20/2012 )
I have read many studies genrerally inactivate the serum used in assays - inactivation at 56 degree for 30 min.
1. Why this is done ?
2. Is this inactivation done only for specific experiments like - Invitro inhibition, ELISA etc ?
Thank you for your time.
Heat inactivation was 1c deemed necessary 2 destroy heat labile components,such as complements.
In addition 2 complement inactivation, heat inactivation was believed 2 inactivate adventitious microbial contaminants such as mycoplasmas...
U can find drawbacks of heat inactivation here:
heat inactivation increases the formation of precipitates (which are frequently mistaken for a microbial contaminant) & decreases d growth promotion capacity of d serum