Melanin labeling - (Oct/31/2012 )
I need to verify melanins in human epidermis. I've prepared some histologic sections, and want to see melanins using confocal microscopy. But, the problem is that there are a lot of bright dots in confocal images, hence recognizing melanins is impossible. So i'm thinking about lableling melanins and imaging in fluoresecence mode. I don't know which antibody and consequently which antigen would be suitable for labeling melanin. Any ideas or suggestions are welcomed.
Thanks in Advance,
did you stain by Fontana-Masson method? that must work well !