Protocol Online logo
Top : New Forum Archives (2009-): : General Biology Discussion

Dilution help for Bradfords Assay - (Oct/19/2012 )

Can someone help me understand why a sample has a final concentration of 1500ug/ml of BSA. If I add 2mg/ml of stock, 10ul of water and 150ul of bradfords reagent.

If someone can point me in the right direction of how they worked it out. Thanks a lot.


what volume of 2mg/ml did you use? by adding water you are diluting it so that you get a reading that is the equivalent of 1500ug/ml (1.5mg/ml) bsa (also, there are different responses with different proteins).