Multiplex PCR - (Oct/17/2012 )
the male and female templates are old whereas other unknown templates were new.
It was stored at 4 degrees because i use it everyday.
-Mad Researcher-
To be sure I'd check the DNA with a gel.
And if you replaced all reagents without success, perhaps the thermal cycler is not working properly, or someone changed the program (accidentally hopefully)?
-hobglobin-
i checked the DNA with the nanodrop though and it was fine, but yes i would check the DNA with the gel also.
As for the thermal cycler program change - i double check it to make sure its the right program.
-Mad Researcher-
hobglobin on Thu Nov 1 16:23:34 2012 said:
To be sure I'd check the DNA with a gel.
And if you replaced all reagents without success, perhaps the thermal cycler is not working properly, or someone changed the program (accidentally hopefully)?
I checked the DNA with gel, they are fine.
-Mad Researcher-