competent cells: CaCl2 autoclave vs filter sterilisation - (Oct/08/2012 )
SF_HK on Tue Oct 9 09:50:27 2012 said:
Ok,
Lets say I make 1M Cacl2 (filter sterilise).
to make 200ml 0.1M Cacl2 (+15 glycerol)
- 20ml 1M CaCl2 (filter sterilised)
-30ml autoclaved 100% glycerol
-170ml autoclaved water
150ml water
-mdfenko-
Thank you. Yes I realised the calculation error when I was preparing the solutions.
-SF_HK-
