competent cells: CaCl2 autoclave vs filter sterilisation - (Oct/08/2012 )
SF_HK on Tue Oct 9 09:50:27 2012 said:
Lets say I make 1M Cacl2 (filter sterilise).
to make 200ml 0.1M Cacl2 (+15 glycerol)
- 20ml 1M CaCl2 (filter sterilised)
-30ml autoclaved 100% glycerol
-170ml autoclaved water
Thank you. Yes I realised the calculation error when I was preparing the solutions.
Hi everyone. Im trying to prepare a 1M CaCl2 to make competent E.coli.
The chemicals available in my lab is anhydrous granular form, laboratory grade.
So my question is:
1. Does granular form of chemicals make accurate measurement of mw compared to powder form?
2. Is laboratory grade cacl2 okay for making competent cells?
Appreciate any random opinions. Thanks