Competitive vs. Sandwich ELISA - (Sep/21/2012 )

Hi!

Due to which reason(s) is a competitive ELISA more sensitive than a sandwich ELISA?

Thank you!

(Source: e.g. http://www.ncbi.nlm.nih.gov/books/NBK92434/#immunometh.J_INITIAL_CONCEPT_AND_METHOD)

-Ventilator-

Hmmm... homework...

-bob1-

-Ventilator-

The general rule with homework is that you need to provide some sort of answer so that we can help you learn, rather than us just providing an answer.

-bob1-

This is not a "homework".

-Ventilator-

Still - it'll help you learn if you can think about the problem and tell us what you think, then we can provide expansion and/or corrections to your thoughts.

-bob1-

A competitive immunoassay is more sensitive to matrix issues. In contrast to sandich, in copetitive elisa the matrix effect contributes to the increasment of sensitivity during the competition step.

-Sotirios-