Competitive vs. Sandwich ELISA - (Sep/21/2012 )
Due to which reason(s) is a competitive ELISA more sensitive than a sandwich ELISA?
(Source: e.g. http://www.ncbi.nlm.nih.gov/books/NBK92434/#immunometh.J_INITIAL_CONCEPT_AND_METHOD)
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A competitive immunoassay is more sensitive to matrix issues. In contrast to sandich, in copetitive elisa the matrix effect contributes to the increasment of sensitivity during the competition step.