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problem with pGL3basic luc activity - (Sep/10/2012 )

I have some problem because the pGL3basic alone (transfected into hela cells) answers to dose dependant assay. I tried with different doses of expression vector for the expression of the cjun protein which repress the basal activity of the pGL3basic.I obtained the same results when I cloned the promoter of my gene. I can't conclude the results of my transfection.I don't know if the repressive effect that I observed with cjun is due to the promoter of my gene or is due to the pGL3basique.
anyone had the same problem with pGL3basique
anyone can help?
thanks

-mcunaj-

pGL3 has relatively high background activity which can be inducible upon stimulation with exogenous agents. I think its because of some kind of cryptic promoter activity. I know that pXPG-Luc has lower background activity (like 20 x lower than pGL3 Basic in HEK293 T) but also I strongly suspect the new version pGL4 Basic is better as well. pXPG is avaialble from Peter Cockerill and pGL4 from Promega.

Here is a useful reference:

http://www.ncbi.nlm.nih.gov/pubmed?term=pXPG%20Cockerill

-srpres-

Thanks for your response . I will try the new version pGL4.

-mcunaj-