Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Cloning

E. coli growing on plates and bot not in LB medium - (Sep/06/2012 )

Pages: Previous 1 2 

I would double check that your 1 liter LB medium is what you think it is. Use the same bottle of the same medium for picking colonies off your plate, so that there is no ambiguity about the composition and antibiotic status of your medium. Use a large enough flask, as ascacioc suggests.

Pages: Previous 1 2