How to identify beta pancreatic cells in dish of heterogenous cells? - (Jul/26/2012 )
Sorry for the late reply. Hadn't come online for a few days. Do you mean the staining works but that Ins-1 cells do not express Zn and that is why you didn't see any red cells? I had done the staining on pancreatic tissues but have never tried it on any of the insulinoma cell lines. I used to work with both INS-1E and NIT-1.
And as far as I remember, I just dissolved the DTZ in distilled water and washed it off with distilled water. Also the staining never took longer than a couple of minutes.