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Where can I find the extinction coefficient of glutathione (GSH)? - (Jul/18/2012 )

I've tried to quantify the GSH level in liver tissue. my calibration curve didn't work so i'm trying to calcuate with epsilon instead.
any ideas?

-nataly-

Are you assaying the glutathione directly or using an indirect assay (e.g. compound that reduces glutathione, measure colour change of said compound)?

-bob1-

I'm using nature protocol: http://www.nature.com/nprot/journal/v1/n6/full/nprot.2006.378.html

-nataly-

So you are actually measuring the change in a compound that is not glutathione - are you sure that you shouldn't be looking for the extinction coefficient for DTNB or TNB?

-bob1-