Where can I find the extinction coefficient of glutathione (GSH)? - (Jul/19/2012 )
I've tried to quantify the GSH level in liver tissue. my calibration curve didn't work so i'm trying to calcuate with epsilon instead.
any ideas?
-nataly-
Are you assaying the glutathione directly or using an indirect assay (e.g. compound that reduces glutathione, measure colour change of said compound)?
-bob1-
I'm using nature protocol: http://www.nature.com/nprot/journal/v1/n6/full/nprot.2006.378.html
-nataly-
So you are actually measuring the change in a compound that is not glutathione - are you sure that you shouldn't be looking for the extinction coefficient for DTNB or TNB?
-bob1-