Inorganic phosphorous - (Jun/30/2012 )
I'm trying out for the quantification of inorganic phosphorus in my samples. I've tried it out with Fiske Subbarow and Munoz et al methods.....But i'm finding it difficult to get consistence reading in both the methods. I know there are number of factors that could have went wrong but I'm zeroing in on improper cleaning of my Test tubes..... can anyone tell me what the best method is to clean the test tubes off inorganic phosphates? Will acid -water do the trick? or any thing else?
we washed with phosphate-free detergent (not really phosphate-free when using sensitive phosphate assay) and rinsed with copious amounts of deionized water.
we measured inorganic phosphate using a method modified from chen, et al, anal. chem., 28: 1756-58 (1956). it is more sensitive than fiske-subbarow.