Uneven lane widths during western blots - (Jun/29/2012 )
mdfenko on Mon Jul 2 15:47:54 2012 said:
as bob1 pointed out, we see this effect when we have different salt concentrations in the samples.
we also see it when there is lipid in the sample.
My salt concentration is 150 mM NaCl and is prepared for both samples (i.e. both samples are homogenized in 100 ul RIPA lysis buffer from the same solution). I don't see how the salt concentration could be different between the two samples then.
Could it be that my RIPA lysis buffer from Millipore is just about to reach its expiration date? http://www.millipore.com/catalogue/item/20-188