Protocol Online logo
Top : New Forum Archives (2009-): : General Lab Techniques

Micrograms from concentration? - (Jun/25/2012 )

Hi there,
I am fairly new to this field, and I have a question about some conversions.
For my research I am using 100ul of a growth factor (of 5ug/ml concentration) in 5 ml of media.

How do i calculate the amount of micrograms I have in the media?

100ul/5000ul is 0.02 ug or 20 nanograms, but how do I use the concentration of the growth factor too while calculating the micrograms?

Thanks a lot, appreciate all the help!


100ul is 1/10th of a ml. it will contain 0.5ug. therefore you will have 0.5ug in 5ml of medium or 0.1ug/ml.