T7 on single stranded promoter sequence? - (May/10/2012 )
Haha, so you are TRYING to do ISH? But not on plants, I assume?
Yes, plants are sometimes terrible to work with... I read some papers that this region was highly repetitive but it's worse than I expected! :|
Yes, a lot of target-enrichment kits work this way; with a probe and than 'fishing' out the probe-bound DNA sequences...
I'm doing ISH on adult mouse olfactory epithelium, its a wonderful system but RNA is super sticky there, so basically you get background regardless of how you wash
Are there any benefits to working on plants?
I'm feeling like c elegans is the best model to work with, if only they had humanoid organs....
Is next gen sequencing of this super necessary for your work? It seems do-able but just barely