Design primers for expression cloning - (Mar/14/2012 )
pmggms2 on Wed Jun 5 20:36:54 2013 said:
Question so why do you add random bps at the beginning of the restriction enzyme
So as to give the RE something to bind to - most of them won't work very well close to the end of a sequence, so you need a few bp (6 is usually enough, some will work with less) beyond the restriction site.
-bob1-
thanks
-pmggms2-