non recombinant Human cytokines - (Mar/09/2012 )
I'm new post graduate student, I'm looking for a piece of advice please:
I'm looking to detect autoantibody in patients serum (if present) against cytokines , by indirect ELISA .
my questions are:
1- where can I get human cytokines of human cell line origin ( not bacteria or plant because the final product is different from human and positive for these doesn't mean it is positive agianst human cytokines) .
2- can you refere any specific reference help me to understand the proper calculation : how much should I add for each well ?
3- can I add more than one cytokines ( say 4 /well) . and then if I have positive for any of the four I added I will do seperate detection for each cytokine and see which one is positive?
other useful points you would like to draw my attention to, will be very appreciated it .
one place may be r&d systems.
Thanks , I sent them an e-mail and wait a reply.