how to find suitable vectors to transform wild-type bacteria - (Mar/07/2012 )
Would you please let me know how to do it? Or how should I search for the protocol or books?
Thanks a lot!
phage434 on Wed Mar 14 18:14:31 2012 said:
Also, you probably should characterize the restriction enzymes present in your organism. Genome analysis may tell you something, but experimenting with cell lysates is the sure way to tell. It would be good to either eliminate restriction sites, or to methylate your DNA to protect it.