Nanodrop3300 - (Feb/15/2012 )
We have a problem measuring our DNA using a NanoDrop3300 fluorospectrometer.
We figured out that with decreasing amounts of DNA to be measured there is a variable named gain increasing at the same time. Does anybody know what this gain exactly stands for? We were told that this is the time how long the sample is measured. With decreasing DNA concentration the sample is exposed for longer times during the measurement. Does anybody know, whether this gain (indicated with numbers ranging from 0.1-10) effects the outcome of the results in any way? Or with other words: can I directly compare 2 DNA where the gain varies from 0.1 – 10?
All helpful comments are more than welcome. Thank you very much in advance!
The gain is a measure of how much "effort" the nanodrop is having to put in to detect the sample, it is not a function of the time taken to measure the sample, rather the time taken is a function of the gain - the machine needs to adjust the gain to measure accurately, and to do that, it must take multiple readings, which means that the time increases.
You can directly compare things with different gains, it is essentially just a way of counteracting the massive signal you would get from a concentrated sample or increasing the signal you would get from a dilute sample.
See this link for more information.