Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Biology

How to prepare Gram(+) bacteria for electrotransformation - (Feb/13/2012 )

Pages: Previous 1 2 

Thanks a lot! I have prepared 3XMIC today, and will see how the transformation works.

phage434 on Mon Mar 5 03:32:35 2012 said:

I would use untransformed cells to test the MIC, then use about 3x MIC in plates for selection. MIC is easy to measure. Infect media with your culture, then aliquot 100 ul into a row of wells on a plate. Add antibiotic to media at a high known concentration, then add 100 ul to the leftmost well. Do 2x serial dilutions across the row, leaving the last well untouched (zero antibiotic). Cover, incubate, and observe growth.

Pages: Previous 1 2