Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Biology

plasmid extraction - (Feb/08/2012 )

Pages: Previous 1 2 

I have another question I am curious about: You let the pellet dry over night. Then it might be very very dry for sure. I usually use a speed vac for a couple of minutes (if I do not have much time) or let it dry for 1-2 h.

What are the consequences if there is a tiny leftover of ethanol? Why is it considered as so bad?


According to Qiagen (from the miniprep handbook), the main consequence of leftover ethanol is that it can interfere with future enzymatic reactions that the DNA will be used in.


But it is not mentioned which ones in particular, like restrictions and PCR reactions, or both?


Any reaction where enzymes will be involved, can be affected with ethanol. So both restriction digests and pcr.

Pages: Previous 1 2