Protocol Online logo
Top : New Forum Archives (2009-): : Tissue and Cell Culture

High glucose for primary astrocyte or neurons? - (Jan/31/2012 )

I have seen that some journals maintain the primary astrocytic or neuronal cultures in high glucose...

Does high glucose concentration means 25mM of glucose or something higher? (for common cell lines, the normal glucose level should be 5mM as I remember...)

Also, for these cultures, is "high glucose" means "normal level" for them? (Since I would like to treat them with high glucose treatment, so need to choose the concentrations now...)


We usually grow primary neuronal cultures in neurobasal media. Check what is the glucose conc. in Neurobasal.

I am not sure about astrocytic culture