Number of snap per hour in time laps system - (Jan/30/2012 )
I'm going to do some time laps experiments using HONE cell line and I'm wondering how many snaps I can take per hour. More precisely I'm wondering how many fluorescence exposition i can do per hour (24h total) to avoid or have small phototoxicity. If you have any average it could be a great help.
Thanks in advance.
It depends on what you are wanting to see - migration of cells? transport of a protein? cell divisons?... The biggest problem with fluorescence is the heat generated by the bulb running, and potential photo-bleaching of your fluorophore with the light running.