DNA Storage - (Jan/23/2012 )
phage434 on Mon Jan 30 14:35:58 2012 said:
This is an unnecessary limitation. Your enzymatic reactions typically use large amounts of magnesium in their buffers. If your DNA sample is a small fraction of your total volume, then the impact of EDTA in the buffer is very small, and in my experience negligible.
I never tested the effect of EDTA, but we don't experience any DNA degradation with current storage conditions. In some cases we neeeded to use a large volume of sample, I had a DNA loss in one sample, that resulted in concentration about 2ng/ul, and put a maximum to PCR, but that of course are extremes.