Protocol Online logo
Top : New Forum Archives (2009-): : General Lab Techniques

HCL instead of imidazole! - (Dec/15/2011 )

Hi there,

Soo today I eluted with MCAC-100 (or so I thought)..I realsied once i added reducing buffer i added 1M HCL (i have them in the same size bottle next to each other on my shelf) what i did was i just washed my column with MCAC-0 a few times and eluted with the correct imidazole concentration ...just wondering will this running as gel as i write this but just wondering if the beads are still useable



or more likely will the HCl remains on the beads affect my protein :(


you may have hydrolyzed your protein. if the exposure was short then you may still have some useful protein. your gel will tell.