HCL instead of imidazole! - (Dec/15/2011 )
Soo today I eluted with MCAC-100 (or so I thought)..I realsied once i added reducing buffer i added 1M HCL (i have them in the same size bottle next to each other on my shelf)...so what i did was i just washed my column with MCAC-0 a few times and eluted with the correct imidazole concentration ...just wondering will this work..im running as gel as i write this but just wondering if the beads are still useable
or more likely will the HCl remains on the beads affect my protein
you may have hydrolyzed your protein. if the exposure was short then you may still have some useful protein. your gel will tell.