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shrinking CM Cellulose - (Nov/28/2011 )

Hi all,
I am facing some problem regarding the shrinking of my matrix ie., CM cellulose , upto the packing of column everyting is ok even when i use my buffer to wash the column everything seems to be alright but the moment i load my samples and try to elute it out using a gradient of Nacl solution ,the matrix starts shrinking even upto 1cm after the run is complete when i wash it with my buffer and keep it overnight without running it ,it swells back upto 0.5 cm .Can anyone help me in this regard



Regards,
Neeta .P

-Neeta-

this is normal. the high salt causes shrinkage of the matrix (some matrices will shrink a lot more).

-mdfenko-