Half-life of bacteriophages in sterile filtered sewage water - (Nov/11/2011 )
I'd say it depends on which types of phage you have in your falcon, but in my experience 2 months in the fridge should be OK. Just don't freeze-thaw them. I think phage are well adapted to surviving for a long time outside cells. I am no phage specialist, but I've done a lot of phage display experiments and the titer of my phage stock in the fridge did not drop noticeably in two months.
Why not run the experiment yourself? A simle plaque assay in stability context should work.
Thanks a lot for the replies. And sorry for the late reply!
The pre-test confirmed plenty of plaques on the agar plates, thus verifying that bacteriophages were present in the sewage plant water sample at my disposal. I later did several plaque assays with it. I also read in a paper about some t-phages that it should be possible to store them for 2 months in the fridge easily.
Phil Geis: I asked because I short of time and wanted a comment about the likelihood of active phages in advance/at the same time of the trials.
Jim (Vector Inoculator)
ps) I usually dont work with phages and bacteria, but inoculate (infect) my own test organisms with viruliferous half-winged insects, as vectors.