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Iipoprotein determination - (Oct/11/2011 )

Hi,

Im currently trying to separate lipoproteins (HDL, VDL, LDL). Once separated is there anyway to determine what I have separated is that particular type of lipoprotein?

I am not in need to quantify the lipoprotein just prove with some degree that it is either HDL, LDL, VLDL.

I shall then dilute the lipoprotein and place them in to a boyden chambre and analyse to see if any THP-1 differentiated macrophages migrate towards them.

Riz

-swizla21-

as it shows in the pictures with your procedure, the bands will be prominent and you'll be able to determine which is which by their position in the gradient.

-mdfenko-

But is there a catagorical way to determine what they are. For example if i took samples off the band and gave them to someone but I did not label them. Could i performa test to one confirm they are lipids and secondly that they are either LDL, HDL etc? for some reason im thing of some sort of electrophoresis procedure!

-swizla21-

the papers referenced (and linked) in one of your other threads show electrophoretic identification of the three lipoproteins.

-mdfenko-