Protocol Online logo
Top : New Forum Archives (2009-): : Immunology

IHC Storing samples - (Sep/25/2011 )

I have some questions regarding storing of samples midway through the protocol. Is it okay to store samples after fixing so that I can stain the next day? If so, should I store it dry or in solution? Would storing it dry affect the quality of the staining? Thanks.


It should be OK to store after fixing - after all fixing is about stopping things happening in the tissue. If you are planning on processing the samples in a short time frame then store them wet in PBS or TBS.

The storage will depend on how they are fixed and what sort of sample it is - cells on a coverslip can be stored in the -20 for a while, but blocks of mounted tissue are best stored in the fridge or at room temperature.


Thanks for your reply, Bob1. I really appreciate it! :).