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Multiplex PCR - (Sep/07/2011 )

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here's a presentation of the problem with the aspecific product,

Attached File


I'm not expert in Real-time PCR, however, I do find that your CT value is quite high, >30
I can see there are some possible primer dimmers in your melting curve in your last slide: is this what you mean by "amplify other products"?

-Adrian K-

yes, but the strange thing is that if you use the primerpair seperated you dont see this product. Probably primer heterodimer between the 2 primerpair, like you say

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