Glycerol stock - (Jul/24/2011 )

I have worked on plasmid DNA isolation and cell retransformation and i was ask to prepare glycerol stock for recombinant strain and how to ensure it is functional of gene expression(refering to glycerol stock).

For preparation of glycerol stock: I chose -70oC with 15% glycerol.

However, i am not sure how to ensure that it is functional.

Any help will be appreciated

Agape on Sun Jul 24 13:48:45 2011 said:

I have worked on plasmid DNA isolation and cell retransformation and i was ask to prepare glycerol stock for recombinant strain and how to ensure it is functional of gene expression(refering to glycerol stock).

For preparation of glycerol stock: I chose -70oC with 15% glycerol.

However, i am not sure how to ensure that it is functional.

Any help will be appreciated

Adrian K on Sun Jul 24 15:36:20 2011 said:

Agape on Sun Jul 24 13:48:45 2011 said:

I have worked on plasmid DNA isolation and cell retransformation and i was ask to prepare glycerol stock for recombinant strain and how to ensure it is functional of gene expression(refering to glycerol stock).

For preparation of glycerol stock: I chose -70oC with 15% glycerol.

However, i am not sure how to ensure that it is functional.

Any help will be appreciated

If your cell is E.coli, is ok.

For functionality, the easiest way is to prepare plasmid from bacteria taken from your glycerol stock, and then express the protein encoded, detect by western blot.