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Dissolving Calcium Chloride in PBS: How to Prevent Precipitation? - (Jul/07/2011 )

Hi there,

I am trying to make PBS containing 100 mM CaCl2. However, when I dissolve the correct amount of CaCl2 in the PBS, there is a formation of precipitate. Does anyone know how to prevent this and make 100 mM CaCl2 PBS?

-Newaj Abdullah-

You are probably forming CaHPO4 which is very insoluble in water... you will have to make a more dilute solution of Ca2+ as you will never be able to overcome this problem.


in addition to bob1: add stock solution of CaCl2 stepwise to PBS under stiring

-Inmost sun-