Genotyping hundreds of samples using SNP microarrays. How to design the assay? - (Jul/07/2011 )
Hi all! I'm a microarray beginner and I'd appreciate your help...
My purpose is to design an association study involving 200 family trios (~600 samples) and investigate a possible association between SNPs/tagSNPs (located in 6 different genes) and Attention Deficit Hiperactivity disorder.
I could use microarrays for genotyping...I don't know which platform I'll use yet but my questions are related with the study design:
- Which methods can I use to label the target DNA, namely each SNP?
- Can I genotype all the 600 samples and analyze all the SNPs simultaneously in a single microarray assay? Each "spot" of the array would correspond to a different sample and withing each spot there are immobilized probes complementar to all the SNPs?
- Please can someone highlight me about how to run a SNP microarray assay in this situation?
Very nooby questions but I'm really confused
I´m new in the forum and only see your post today...... but do you already start?