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Dark western blot film - (Jul/04/2011 )

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Yes, I washed it with PBST


I've sometimes get that with the Amersham ECL Advance Kit, I've found it to be very sensitive and gives a very high background, I see bands that are unspecific which I dont see with other kits, and sometimes I even seen the marker bands... overall I get very blank backgrounds...

I suggest you try another substrate kit for HRP and carefully dry the membrane of excess substrate...


you should better wash twice with PBS without triton or tween before to put the substrate.

-little mouse-
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